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pcDNA3-LAP2-YFP-NLS
(Plasmid #25847)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25847 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LAP2-YFP-NLS
  • Alt name
    Coumarin PRIME positive control plasmid
  • Insert Size (bp)
    867
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-LAP2-YFP-NLS was a gift from Alice Ting (Addgene plasmid # 25847 ; http://n2t.net/addgene:25847 ; RRID:Addgene_25847)

  • For your References section:

    A fluorophore ligase for site-specific protein labeling inside living cells. Uttamapinant C, White KA, Baruah H, Thompson S, Fernandez-Suarez M, Puthenveetil S, Ting AY. Proc Natl Acad Sci U S A. 2010 Jun 15. 107(24):10914-9. 10.1073/pnas.0914067107 PubMed 20534555
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