pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase
(Plasmid #25846)

• Depositing Lab: Alice Ting
• Publication: Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 107(24):10914-9.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LplA-W37I
• Alt name: Alternative coumarin PRIME ligase
• Alt name: Less sensitive, more specific
• Species: E. Coli
• Insert Size (bp): 1042
• Mutation: W37I
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original LplA gene a gift from John Cronan (Univ. of Illinois)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The original E. coli LplA gene from John Cronan was resynthesized using codons optimal for mammalian expression.