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Addgene

pfmh-hCry1
(Plasmid #25843)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25843 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4
  • Backbone size w/o insert (bp) 5274
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Cryptochrome 1
  • Alt name
    hCry1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1757
  • Entrez Gene
    CRY1 (a.k.a. DSPD, PHLL1)
  • Tags / Fusion Proteins
    • flag (C terminal on insert)
    • myc (C terminal on backbone)
    • his (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (unknown if destroyed)
  • 3′ cloning site BstBI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pfmh-hCry1 was a gift from Aziz Sancar (Addgene plasmid # 25843 ; http://n2t.net/addgene:25843 ; RRID:Addgene_25843)
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