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Addgene

pMal672H
(Plasmid #25817)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25817 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST 14
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 6700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rap1(672-827)
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    468
  • GenBank ID
    NP_014183
  • Entrez Gene
    RAP1 (a.k.a. YNL216W, GRC4, GRF1, TBA1, TUF1)
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • His6 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMal672H was a gift from Cynthia Wolberger (Addgene plasmid # 25817 ; http://n2t.net/addgene:25817 ; RRID:Addgene_25817)

  • For your References section:

    Structural and functional studies of the Rap1 C-terminus reveal novel separation-of-function mutants. Feeser EA, Wolberger C. J Mol Biol. 2008 Jul 11. 380(3):520-31. 10.1016/j.jmb.2008.04.078 PubMed 18538788
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