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TU#867 (Punc-119sid-1)
(Plasmid #25811)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25811 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD95.75
  • Backbone manufacturer
    A. Fire
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    systemic RNA interference-deficient 1
  • Alt name
    sid-1
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    7860
  • Entrez Gene
    sid-1 (a.k.a. CELE_C04F5.1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site PvuI (not destroyed)
  • 5′ sequencing primer TATAGGATCCTCATTTTTCCAGGTTCACAATG
  • 3′ sequencing primer ATATAAGCGGCCGCAGAAAGGTGTCATGGTCTAGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Specific details about this vector are given at the Materials and Methods of the article: Calixto et al, 2010, Nat Meth "Enhanced Neuronal RNAi using sid-1"

Note that this plasmid contains genomic sid-1, NOT the cDNA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TU#867 (Punc-119sid-1) was a gift from Martin Chalfie (Addgene plasmid # 25811 ; http://n2t.net/addgene:25811 ; RRID:Addgene_25811)

  • For your References section:

    Enhanced neuronal RNAi in C. elegans using SID-1. Calixto A, Chelur D, Topalidou I, Chen X, Chalfie M. Nat Methods. 2010 May 30. ():. 10.1038/nmeth.1463 PubMed 20512143
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