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Addgene

pcDNA3-HA-PQBP1
(Plasmid #25804)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25804 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PQBP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • GenBank ID
    BC012358
  • Entrez Gene
    PQBP1 (a.k.a. MRX2, MRX55, MRXS3, MRXS8, NPW38, RENS1, SHS)
  • Tag / Fusion Protein
    • 6xHA (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Mathieu Bollen, Co-author. Dr. Erica A. Golemis, Collaborator.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Several references within the article

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-PQBP1 was a gift from Marius Sudol (Addgene plasmid # 25804 ; http://n2t.net/addgene:25804 ; RRID:Addgene_25804)

  • For your References section:

    Y65C missense mutation in the WW domain of the golabi-ito-hall syndrome protein PQBP1 affects its binding activity and deregulates pre-mRNA splicing. Tapia VE, Nicolaescu E, McDonald CB, Musi V, Oka T, Inayoshi Y, Satteson AC, Mazack V, Humbert J, Gaffney CJ, Beullens M, Schwartz CE, Landgraf C, Volkmer R, Pastore A, Farooq A, Bollen M, Sudol M. J Biol Chem. 2010 Apr 21. ():. 10.1074/jbc.M109.084525 PubMed 20410308
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