pcDNA4-His-Max-A-PQBP1
(Plasmid
#25802)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 25802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA4-His-MaxA
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5300
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePQBP1
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1000
-
GenBank IDBC012358
-
Entrez GenePQBP1 (a.k.a. MRX2, MRX55, MRXS3, MRXS8, NPW38, RENS1, SHS)
-
Tags
/ Fusion Proteins
- His (N terminal on insert)
- Xpress (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (unknown if destroyed)
- 3′ cloning site EcoR1 (unknown if destroyed)
- 5′ sequencing primer n/A
- 3′ sequencing primer n/A (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA4-His-Max-A-PQBP1 was a gift from Marius Sudol (Addgene plasmid # 25802 ; http://n2t.net/addgene:25802 ; RRID:Addgene_25802) -
For your References section:
Y65C missense mutation in the WW domain of the golabi-ito-hall syndrome protein PQBP1 affects its binding activity and deregulates pre-mRNA splicing. Tapia VE, Nicolaescu E, McDonald CB, Musi V, Oka T, Inayoshi Y, Satteson AC, Mazack V, Humbert J, Gaffney CJ, Beullens M, Schwartz CE, Landgraf C, Volkmer R, Pastore A, Farooq A, Bollen M, Sudol M. J Biol Chem. 2010 Apr 21. ():. 10.1074/jbc.M109.084525 PubMed 20410308