pGL3-PMT-CD41
(Plasmid #25801)

• Depositing Lab: Judy Lieberman
• Publication: Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: promega
• Backbone size w/o insert (bp): 4818
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CD41 promoter
• Insert Size (bp): 627
• Tag / Fusion Protein:
  • luciferase (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence of the promoter region of CD41 corresponds to positions -597 to +32 of the human GPIIb promoter (full length promoter) described in Sevinsky et al., Mol. Cell. Biol. 2004, 24(10):4534-4545.