pGL3-PM-34a-K1/2
(Plasmid #25800)

• Depositing Lab: Judy Lieberman
• Publication: Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: promega
• Backbone size w/o insert (bp): 4818
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alternative hsa-miR-34a promoter
• Alt name: alternative pri-miR34a transcript
• Alt name: pri-miR-34a-K1/2
• Insert Size (bp): 2013
• Tag / Fusion Protein:
  • luciferase (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

promoter region of the alternative pri-miR-34a transcript identified by Navarro et al. Blood 2009 Sep 3;114(10):2181-92.

There are several discrepancies between this sequence and the GenBank sequence: T(at position 388) is cahnged to C

"ACACAC" insertion at position 658

"T" insertion at position 1914

A (at position 1599) changed to G

T (at position 1480) changed to G

G (at position 1212) changed to A

T (at position 1199) changed to C

These changes were present in all the clones that the lab sequenced and these clones came from two independent PCRs using genomic DNA extracted from the human erithroleukemia cell line K562. The changes might be due to that particular cell line. Importantly, all the minipreps I tested induced luciferase activity in K562 cells upon TPA treatment (as reported in the manuscript).