pGL3-MYB-3'UTR
(Plasmid #25798)

• Depositing Lab: Judy Lieberman
• Publication: Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Backbone

• Vector backbone: pGL3-control
• Backbone manufacturer: promega
• Backbone size w/o insert (bp): 5256
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3'UTR of human MYB
• Alt name: c-myb
• Alt name: 3'UTR MYB
• Species: H. sapiens (human)
• Insert Size (bp): 1191
• GenBank ID: NM_005375
• Entrez Gene: MYB (a.k.a. Cmyb, c-myb, c-myb_CDS, efg)
• Tag / Fusion Protein:
  • luciferase (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The c-myb 3'UTR sequence replaces the SV40 enhancer and SV40 poly(A) signal of pGL3-control