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pSIREN-RetroQ-CDK6-shRNA
(Plasmid #25789)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25789 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSIREN-RetroQ
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRNA against human CDK6
  • Alt name
    shRNA CDK6
  • Alt name
    CDK6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    53
  • GenBank ID
    NM_001259
  • Entrez Gene
    CDK6 (a.k.a. MCPH12, PLSTIRE)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer GGGCAGGAAGAGGGCCTAT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIREN-RetroQ-CDK6-shRNA was a gift from Judy Lieberman (Addgene plasmid # 25789 ; http://n2t.net/addgene:25789 ; RRID:Addgene_25789)

  • For your References section:

    miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53. Navarro F, Gutman D, Meire E, Caceres M, Rigoutsos I, Bentwich Z, Lieberman J. Blood. 2009 Sep 3. 114(10):2181-92. 10.1182/blood-2009-02-205062 PubMed 19584398
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