pCS2 xenopus Insulin 2
(Plasmid
#25786)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25786 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2
- Backbone size w/o insert (bp) 4095
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameInsulin 2
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SpeciesX. laevis (frog)
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Insert Size (bp)340
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Antisense: cut with EcoRI, transcribe with T7
Sense: cut with NotI, transcribe with SP6
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2 xenopus Insulin 2 was a gift from Edward De Robertis (Addgene plasmid # 25786 ; http://n2t.net/addgene:25786 ; RRID:Addgene_25786) -
For your References section:
Isthmin is a novel secreted protein expressed as part of the Fgf-8 synexpression group in the Xenopus midbrain-hindbrain organizer. Pera EM, Kim JI, Martinez SL, Brechner M, Li SY, Wessely O, De Robertis EM. Mech Dev. 2002 Aug . 116(1-2):169-72. 10.1016/S0925-4773(02)00123-5 PubMed 12128218
Map uploaded by the depositor.
