pGEM-T-easy Sox17b
(Plasmid
#25782)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25782 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-T Easy
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3015
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSox 17b
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Alt nameSox17b
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SpeciesX. laevis (frog)
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Entrez Genesox17b.1 (a.k.a. Sox17beta, XSox17beta, Xsox17, sox17, sox17-beta, sox17b, sox17b.1-a, tSox17beta, xsox17-beta)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ApaI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For antisense RNA cut with ApaI, transcribe with SP6
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-T-easy Sox17b was a gift from Edward De Robertis (Addgene plasmid # 25782 ; http://n2t.net/addgene:25782 ; RRID:Addgene_25782) -
For your References section:
The Xenopus homologue of Bicaudal-C is a localized maternal mRNA that can induce endoderm formation. Wessely O, De Robertis EM. Development. 2000 May . 127(10):2053-62. PubMed 10769230