pCS2 mouse CV2 PC
(Plasmid #25776)

• Depositing Lab: Edward De Robertis
• Publication: Coffinier et al Mech Dev. 2002 Dec . 119 Suppl 1():S179-84.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4095
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cross veinless 2
• Alt name: CV2
• Species: M. musculus (mouse)
• Insert Size (bp): 2300
• Entrez Gene: Bmper (a.k.a. 3110056H04Rik, CV-, CV-2, Cri, Crim3, Cv, Cv2)
• Tag / Fusion Protein:
  • PC (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For sense, linearize with NotI, transcribe with SP6.

Protein C Epitope tag sequence - EDQVDPRLIDGK