pBS Zpapc
(Plasmid
#25774)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 25774 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript-SK
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePapc
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)3000
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Entrez Genepcdh8 (a.k.a. papc, zPAPC)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
antisense: linearize with Apa1, transcribe with T3
sense: linearize with Not1, transcribe with T7
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS Zpapc was a gift from Edward De Robertis (Addgene plasmid # 25774 ; http://n2t.net/addgene:25774 ; RRID:Addgene_25774) -
For your References section:
Zebrafish paraxial protocadherin is a downstream target of spadetail involved in morphogenesis of gastrula mesoderm. Yamamoto A, Amacher SL, Kim SH, Geissert D, Kimmel CB, De Robertis EM. Development. 1998 Sep . 125(17):3389-97. PubMed 9693142
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/74/98/52e76688-af63-11e0-90fe-003048dd6500.jpeg.940x940_q85_autocrop.png)