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Addgene

pBS Zpapc
(Plasmid #25774)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25774 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript-SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Papc
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    3000
  • Entrez Gene
    pcdh8 (a.k.a. papc, zPAPC)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

antisense: linearize with Apa1, transcribe with T3

sense: linearize with Not1, transcribe with T7

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS Zpapc was a gift from Edward De Robertis (Addgene plasmid # 25774 ; http://n2t.net/addgene:25774 ; RRID:Addgene_25774)

  • For your References section:

    Zebrafish paraxial protocadherin is a downstream target of spadetail involved in morphogenesis of gastrula mesoderm. Yamamoto A, Amacher SL, Kim SH, Geissert D, Kimmel CB, De Robertis EM. Development. 1998 Sep . 125(17):3389-97. PubMed 9693142
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