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Addgene

pFAST NPC1(NTD)-3Q
(Plasmid #25772)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25772 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFASTBAC
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    npc1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    687
  • Mutation
    aa23-252 only; N70Q N122Q N185Q
  • Entrez Gene
    NPC1 (a.k.a. NPC, POGZ, SLC65A1)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SFO1 (not destroyed)
  • 3′ cloning site ECOR1 (not destroyed)
  • 5′ sequencing primer Polyhedrin forward
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a TEV cleavage site downstream of His tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFAST NPC1(NTD)-3Q was a gift from Johann Deisenhofer (Addgene plasmid # 25772 ; http://n2t.net/addgene:25772 ; RRID:Addgene_25772)

  • For your References section:

    Structure of N-terminal domain of NPC1 reveals distinct subdomains for binding and transfer of cholesterol. Kwon HJ, Abi-Mosleh L, Wang ML, Deisenhofer J, Goldstein JL, Brown MS, Infante RE. Cell. 2009 Jun 26. 137(7):1213-24. 10.1016/j.cell.2009.03.049 PubMed 19563754
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