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pGEX-EGFAB(H306Y)
(Plasmid #25771)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25771 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LDL receptor EGFAB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    240
  • Mutation
    His 306 to Tyr
  • Entrez Gene
    LDLR (a.k.a. FH, FHC, FHCL1, LDLCQ2)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NCO1 (not destroyed)
  • 3′ cloning site ECOR1 (not destroyed)
  • 5′ sequencing primer pGEX 5'
  • 3′ sequencing primer pGEX 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was generated in the Deisenhofer lab but first described in J Biol Chem. 2009 Apr 17. 284(16):10561-70.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-EGFAB(H306Y) was a gift from Johann Deisenhofer (Addgene plasmid # 25771 ; http://n2t.net/addgene:25771 ; RRID:Addgene_25771)

  • For your References section:

    Antagonism of secreted PCSK9 increases low density lipoprotein receptor expression in HepG2 cells. McNutt MC, Kwon HJ, Chen C, Chen JR, Horton JD, Lagace TA. J Biol Chem. 2009 Apr 17. 284(16):10561-70. 10.1074/jbc.M808802200 PubMed 19224862
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