pGEX-EGFAB
(Plasmid #25770)

• Depositing Lab: Johann Deisenhofer
• Publication: Kwon et al Proc Natl Acad Sci U S A. 2008 Feb 12. 105(6):1820-5.

Backbone

• Vector backbone: pGEX
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LDL receptor EGFAB
• Species: H. sapiens (human)
• Insert Size (bp): 240
• Entrez Gene: LDLR (a.k.a. FH, FHC, FHCL1, LDLCQ2)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NCO1 (not destroyed)
• 3′ cloning site: ECOR1 (not destroyed)
• 5′ sequencing primer: pGEX 5'
• 3′ sequencing primer: pGEX 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry