pSLIK-Venus-TmiR-G13-G12
(Plasmid #25742)

• Purpose: Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.
• Depositing Lab: Iain Fraser
• Publication: Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 103(37):13759-64.

Backbone

• Vector backbone: pSLIK
• Backbone manufacturer: Iain Fraser
• Backbone size w/o insert (bp): 12752
• Vector type: Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: G alpha 13 and 12 miR-shRNA
• Species: M. musculus (mouse)
• Tag / Fusion Protein:
  • Venus (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EXFP-R, hUBCpro-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: rtTA- Atze Das, University of Amsterdam; TRE promoter- David Anderson, Caltech; miR30- Greg Hannon, CSHL Venus- Tobias Meyer, Stanford

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSLIK-Venus with G alpha 12 and 13 miR-shRNA

G alpha 12 miR-shRNA: GCGACACCATCTTCGACAACAT

G alpha 13 miR-shRNA: CTGGGTGAGTCTGTAAAGTATT

There are 3 minor changes between depositor's sequence and Addgene's sequence - An extra T between the TRE promoter and first shRNA; a 17nt stretch between the shRNAs is different but will not affect function; and a single nt change mutates an EcoRI site just beyond the 2nd shRNA but will again not affect function.