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Addgene

piRES-puro-ELF3
(Plasmid #25728)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESpuro2
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E74-like factor 3 (ets domain transcription factor, epithelial-specific )
  • Alt name
    ELF3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1115
  • GenBank ID
    NM_001114309
  • Entrez Gene
    ELF3 (a.k.a. EPR-1, ERT, ESE-1, ESX)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer sequence
  • 3′ sequencing primer sequence
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piRES-puro-ELF3 was a gift from Ronny Drapkin (Addgene plasmid # 25728 ; http://n2t.net/addgene:25728 ; RRID:Addgene_25728)
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