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Addgene

piRES-puro-HE4
(Plasmid #25727)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25727 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESpuro2
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    WAP four-disulfide core domain 2
  • Alt name
    WFDC2
  • Alt name
    HE4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    374
  • Mutation
    N/A
  • GenBank ID
    NM_006103
  • Entrez Gene
    WFDC2 (a.k.a. EDDM4, HE4, WAP5, dJ461P17.6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer sequence
  • 3′ sequencing primer sequence
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piRES-puro-HE4 was a gift from Ronny Drapkin (Addgene plasmid # 25727 ; http://n2t.net/addgene:25727 ; RRID:Addgene_25727)
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