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Addgene

pcDNA FLAG TORC1 (1-634)
(Plasmid #25718)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25718 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.0
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TORC1 (1-634)
  • Alt name
    TORC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2000
  • Entrez Gene
    CRTC1 (a.k.a. MAML2, MECT1, Mam-2, TORC-1, TORC1, WAMTP1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA FLAG TORC1 (1-634) was a gift from Marc Montminy (Addgene plasmid # 25718 ; http://n2t.net/addgene:25718 ; RRID:Addgene_25718)

  • For your References section:

    TORCs: transducers of regulated CREB activity. Conkright MD, Canettieri G, Screaton R, Guzman E, Miraglia L, Hogenesch JB, Montminy M. Mol Cell. 2003 Aug . 12(2):413-23. 10.1016/j.molcel.2003.08.013 PubMed 14536081
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