pAV U6+27
(Plasmid #25709)

• Purpose: for nuclear expression of small RNAs in mammalian cells
• Depositing Lab: David Engelke
• Publication: Good et al Gene Ther. 1997 Jan . 4(1):45-54.

Backbone

• Vector backbone: pAV
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): JM105
• Growth instructions: Provided in JM105
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: U6 snRNP
• Alt name: U6
• Species: H. sapiens (human)
• Insert Size (bp): 292
• Entrez Gene: LSM2 (a.k.a. C6orf28, G7B, YBL026W, snRNP)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: hU6-F (5'-GAGGGCCTATTTCCCATGATT-3')
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pAV U6+27 cassette
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The U6+27 cassette was constructed by PCR amplification from 265 nucleotides upstream of the human U6 gene transcription initiation site through the first 27 nucleotides of the transcribed gene.