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200 bp Axin2 mutant
(Plasmid #25704)

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Item Catalog # Description Quantity Price (USD)
Plasmid 25704 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Axin 2 promoter
  • Alt name
    AX2(1078MUT)/Luc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    TCF DNA binding site at bp -108 to -102 mutated CTTTGAT --> CTTTGGC
  • Entrez Gene
    AXIN2 (a.k.a. AXIL, ODCRCS)
  • Tag / Fusion Protein
    • Luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NheI (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains AXIN2 sequences from -181 to +5 relative to the presumed transcription start site.

Mutation listed above.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    200 bp Axin2 mutant was a gift from Eric Fearon (Addgene plasmid # 25704 ; http://n2t.net/addgene:25704 ; RRID:Addgene_25704)

  • For your References section:

    Activation of AXIN2 expression by beta-catenin-T cell factor. A feedback repressor pathway regulating Wnt signaling. Leung JY, Kolligs FT, Wu R, Zhai Y, Kuick R, Hanash S, Cho KR, Fearon ER. J Biol Chem. 2002 Jun 14. 277(24):21657-65. 10.1074/jbc.M200139200 PubMed 11940574
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