IRS1-A4
(Plasmid
#25697)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSUPERIOR RETRO-PURO
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Backbone manufacturerOligoengine
- Backbone size w/o insert (bp) 6349
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Vector typeRetroviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIRS1
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SpeciesH. sapiens (human)
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Entrez GeneIRS1 (a.k.a. HIRS-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN5'
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
sense 5′-GAT CCC GAG CAT GTA CAA ATG CTT CTC TTC CTG TCA AGA AGC GTT TGT GCA TGC TCT TTT TA-3′
antisense 5′-AGC TTA AAA AGA GCA TGC ACA AAC GCT TCT TGA CAG GAA GAG AAG CAT TTG TAC ATG CTC GG
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IRS1-A4 was a gift from Eric Fearon (Addgene plasmid # 25697 ; http://n2t.net/addgene:25697 ; RRID:Addgene_25697) -
For your References section:
IRS1 regulation by Wnt/beta-catenin signaling and varied contribution of IRS1 to the neoplastic phenotype. Bommer GT, Feng Y, Iura A, Giordano TJ, Kuick R, Kadikoy H, Sikorski D, Wu R, Cho KR, Fearon ER. J Biol Chem. 2010 Jan 15. 285(3):1928-38. 10.1074/jbc.M109.060319 PubMed 19843521