pPGS-hSLUG.fl.flag
(Plasmid #25696)

• Depositing Lab: Eric Fearon
• Publication: Slug plasmid (unpublished)

Backbone

• Vector backbone: pPGS-CMV-CITE-Neo(-)
• Backbone manufacturer: Gary Nabel
• Backbone size w/o insert (bp): 7131
• Vector type: Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SLUG
• Species: H. sapiens (human)
• Insert Size (bp): 850
• Entrez Gene: SNAI2 (a.k.a. SLUG, SLUGH, SLUGH1, SNAIL2, WS2D)
• Tag / Fusion Protein:
  • FLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pPGS-CMV-CITE-neo vector allows the expression of chimeric transcripts encoding a gene of interest fused to the neomycin resistance gene. Expression of the neomycin resistance protein results from an internal ribosome entry site upstream of the neomycin open reading frame.