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pEBB XIAP W310A
(Plasmid #25677)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25677 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBB
  • Backbone manufacturer
    Baltimore Lab
  • Backbone size w/o insert (bp) 5353
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XIAP
  • Alt name
    hILP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    W310A
  • GenBank ID
    HSU32974
  • Entrez Gene
    XIAP (a.k.a. API3, BIRC4, IAP-3, ILP1, MIHA, XLP2, hIAP-3, hIAP3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer EF-1a Forward
  • 3′ sequencing primer M13_puc_F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Site-directed mutagenesis was used to change W310 to Ala (TGG to GCG). This mutation disrupts caspase 9 and Smac binding to XIAP.

#717

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBB XIAP W310A was a gift from Colin Duckett (Addgene plasmid # 25677 ; http://n2t.net/addgene:25677 ; RRID:Addgene_25677)

  • For your References section:

    Uncoupling of the signaling and caspase-inhibitory properties of X-linked inhibitor of apoptosis. Lewis J, Burstein E, Reffey SB, Bratton SB, Roberts AB, Duckett CS. J Biol Chem. 2004 Mar 5. 279(10):9023-9. 10.1074/jbc.M312891200 PubMed 14701799
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