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Addgene

pCDF1-p18INK4c
(Plasmid #25650)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25650 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCDF1-MCS2-EF1-Puro
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 6606
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p18INK4c
  • Alt name
    CDKN2C
  • Alt name
    p18
  • Alt name
    INK4c
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1350
  • GenBank ID
    NM_001262
  • Entrez Gene
    CDKN2C (a.k.a. INK4C, p18, p18-INK4C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer atccacgctgttttgacctc
  • 3′ sequencing primer ccaacttctcggggactgt
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDF1-p18INK4c was a gift from Todd Waldman (Addgene plasmid # 25650 ; http://n2t.net/addgene:25650 ; RRID:Addgene_25650)
  • For your References section:

    Identification of p18 INK4c as a tumor suppressor gene in glioblastoma multiforme. Solomon DA, Kim JS, Jenkins S, Ressom H, Huang M, Coppa N, Mabanta L, Bigner D, Yan H, Jean W, Waldman T. Cancer Res. 2008 Apr 15. 68(8):2564-9. 10.1158/0008-5472.CAN-07-6388 PubMed 18381405