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Addgene

pCDF1-PTPRD-WT
(Plasmid #25642)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25642 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCDF1-MCS2-EF1-Puro
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 6606
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Protein tyrosine phosphatase receptor-type delta
  • Alt name
    PTPRD
  • Alt name
    PTP delta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5140
  • Mutation
    An NsiI restriction site was engineered into the multiple cloning site of the pCDF1-MCS2-EF1-Puro vector by site-directed mutagenesis. The PTPRD cDNA with flanking NsiI restriction sites was then cloned into this NsiI site in pCDF1.
  • GenBank ID
    BC106714
  • Entrez Gene
    PTPRD (a.k.a. HPTP, HPTPD, HPTPDELTA, PTPD, R-PTP-delta, RPTPDELTA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NsiI (not destroyed)
  • 3′ cloning site NsiI (not destroyed)
  • 5′ sequencing primer atccacgctgttttgacctc
  • 3′ sequencing primer ccaacttctcggggactgt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDF1-PTPRD-WT was a gift from Todd Waldman (Addgene plasmid # 25642)

  • For your References section:

    Mutational inactivation of PTPRD in glioblastoma multiforme and malignant melanoma. Solomon DA, Kim JS, Cronin JC, Sibenaller Z, Ryken T, Rosenberg SA, Ressom H, Jean W, Bigner D, Yan H, Samuels Y, Waldman T. Cancer Res. 2008 Dec 15. 68(24):10300-6. 10.1158/0008-5472.CAN-08-3272 PubMed 19074898
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