pLKO-RB1-shRNA19
(Plasmid #25640)

• Purpose: 3rd generation lentiviral vector
• Depositing Lab: Todd Waldman
• Publication: Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Backbone

• Vector backbone: pLKO.1 puro
• Backbone size w/o insert (bp): 7032
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RB1-shRNA 19
• Alt name: Retinoblastoma tumor susceptibility protein
• Alt name: RB1
• Alt name: Rb
• Species: H. sapiens (human)
• Insert Size (bp): 42
• GenBank ID: NM_000321
• Entrez Gene: RB1 (a.k.a. OSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LKO.1 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The RNAi Consortium (TRC), MISSION shRNA
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry