pLKO-PTEN-shRNA-3001
(Plasmid #25639)

• Depositing Lab: Todd Waldman
• Publication: Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Backbone

• Vector backbone: pLKO.1 puro
• Backbone size w/o insert (bp): 7032
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PTEN-shRNA-3001
• gRNA/shRNA sequence: CCGGCCACAAATGAAGGGATATAAACTCGAGTTTATATCCCTTCATTTGTGGTTTTT
• Species: H. sapiens (human)
• Insert Size (bp): 42
• GenBank ID: NM_000314
• Entrez Gene: PTEN (a.k.a. 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, PTENbeta, TEP1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LKO.1 5'

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The RNAi Consortium (TRC), MISSION shRNA
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry