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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePET28a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5369
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNampt
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SpeciesM. musculus (mouse)
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Insert Size (bp)1476
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GenBank IDNP_067499
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Entrez GeneNampt (a.k.a. 1110035O14Rik, AI314458, AI480535, NAmPRTase, Pbef, Pbef1, Visfatin)
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Tag
/ Fusion Protein
- His6 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Nampt-PET28a was a gift from Cynthia Wolberger (Addgene plasmid # 25630 ; http://n2t.net/addgene:25630 ; RRID:Addgene_25630) -
For your References section:
Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme. Wang T, Zhang X, Bheda P, Revollo JR, Imai S, Wolberger C. Nat Struct Mol Biol. 2006 Jul . 13(7):661-2. 10.1038/nsmb1114 PubMed 16783373