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pGL3E-hIL-17prom(-159)-Luc
(Plasmid #25629)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25629 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3-Enhancer
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5064
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human IL-17 promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    159
  • Entrez Gene
    IL17A (a.k.a. CTLA-8, CTLA8, IL-17, IL-17A, IL17, ILA17)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Mlu I (not destroyed)
  • 3′ cloning site Hind III (not destroyed)
  • 5′ sequencing primer RVprimer 3
  • 3′ sequencing primer GLprimer2
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3E-hIL-17prom(-159)-Luc was a gift from Sarah Gaffen (Addgene plasmid # 25629 ; http://n2t.net/addgene:25629 ; RRID:Addgene_25629)

  • For your References section:

    Crucial role for nuclear factor of activated T cells in T cell receptor-mediated regulation of human interleukin-17. Liu XK, Lin X, Gaffen SL. J Biol Chem. 2004 Dec 10. 279(50):52762-71. 10.1074/jbc.M405764200 PubMed 15459204
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