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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 25626 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
- Backbone size w/o insert (bp) 4300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameJC Virus Mad-1
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SpeciesHuman polyomavirus
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Insert Size (bp)5000
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (unknown if destroyed)
- 3′ cloning site Bam HI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JC Mad-1 was a gift from Peter Howley (Addgene plasmid # 25626 ; http://n2t.net/addgene:25626 ; RRID:Addgene_25626) -
For your References section:
Cloned human polyomavirus JC DNA can transform human amnion cells. Howley PM, Rentier-Delrue F, Heilman CA, Law MF, Chowdhury K, Israel MA, Takemoto KK. J Virol. 1980 Dec . 36(3):878-82. PubMed 6257931