PRKCH
(Plasmid #25616)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PRKCH
• Alt name: PDB: 2FK9
• Alt name: protein kinase C, eta, C2 domain
• Alt name: MGC26269, MGC5363, nPKC-eta, PKC-L, PKCL, PRKCL
• Species: H. sapiens (human)
• Insert Size (bp): 414
• Mutation: C2 domain (amino acid residues 1-138)
• GenBank ID: BC037268
• Entrez Gene: PRKCH (a.k.a. PKC-L, PKCL, PRKCL, nPKC-eta)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 2FK9 http://www.thesgc.org/structures/2FK9/