NIRF
(Plasmid #25602)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: NIRF
• Alt name: PDB: 1Z6U
• Alt name: Np95-like Ring Finger Protein Isoform B
• Alt name: DKFZp434B0920, DKFZp686G0837, MGC33463, NIRF, RNF107, RP11-472F14.2, URF2
• Species: H. sapiens (human)
• Insert Size (bp): 396
• Mutation: Contains Ring finger domain
• Entrez Gene: UHRF2 (a.k.a. NIRF, RNF107, TDRD23, URF2)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 1Z6U http://www.thesgc.org/structures/1Z6U/