PAPSS2
(Plasmid #25539)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PAPSS2
• Alt name: PDB: 2AX4
• Alt name: 3’-phosphoadenosine-5’-phosphosulfate synthetase, ATP kinase domain
• Alt name: ATPSK2, SK2
• Species: H. sapiens (human)
• Insert Size (bp): 603
• Mutation: Contains kinase domain (residues 21-218)
• GenBank ID: BC009894
• Entrez Gene: PAPSS2 (a.k.a. ATPSK2, BCYM4, SK2)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB:2AX4 http://www.thesgc.org/structures/2AX4/