RAB14
(Plasmid
#25519)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25519 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepET28a-LIC
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Backbone manufacturerSGC
- Backbone size w/o insert (bp) 7328
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRAB14
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Alt namePDB: 4DRZ (supersedes 2AED)
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Alt nameRAB14
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Alt nameFBP, RAB-14
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SpeciesH. sapiens (human)
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Insert Size (bp)531
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Entrez GeneRAB14 (a.k.a. FBP, RAB-14)
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Tag
/ Fusion Protein
- His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is a E71G amino acid change compared to NCBI sequence for 2AED.
PDB ID: 4DRZ (supersedes 2AED) https://www.rcsb.org/structure/4DRZ
SGC link: http://www.thesgc.org/structures/2AED/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RAB14 was a gift from Cheryl Arrowsmith (Addgene plasmid # 25519 ; http://n2t.net/addgene:25519 ; RRID:Addgene_25519)