HRMT1L3
(Plasmid #25508)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HRMT1L3
• Alt name: HMT1 hnRNP methyltransferase-like 3
• Alt name: PDB: 2FYT, 8G2F
• Alt name: HRMT1L3:APC035-H01:C21507
• Species: H. sapiens (human)
• Insert Size (bp): 963
• Mutation: contains amino acids 149-463 only
• Entrez Gene: PRMT3 (a.k.a. HRMT1L3)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 2FYT http://www.thesgc.org/structures/2FYT/
PDB: 8G2F https://www.rcsb.org/structure/8G2F/