EHMT1 (2IGQ)
(Plasmid #25504)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EHMT1
• Alt name: PDB: 2IGQ, 5TTG, 5V9J
• Alt name: euchromatic histone methyltransferase 1
• Species: H. sapiens (human)
• Insert Size (bp): 855
• Mutation: C-terminal domain, residues 951-1235
• Entrez Gene: EHMT1 (a.k.a. EHMT1-IT1, EUHMTASE1, Eu-HMTase1, FP13812, GLP, GLP1, KLEFS1, KMT1D)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 2IGQ http://www.thesgc.org/structures/2IGQ/
PDB: 5TTG http://www.thesgc.org/structures/5TTG/
PDB: 5V9J http://www.thesgc.org/structures/5V9J/