EHMT2
(Plasmid #25503)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: EHMT2
• Alt name: PDB: 2O8J
• Alt name: euchromatic histone-lysine N-methyltransferase 2
• Alt name: BAT8, C6orf30, DKFZp686H08213, FLJ35547, G9A, KMT1C, NG36, NG36/G9a
• Species: H. sapiens (human)
• Insert Size (bp): 843
• Entrez Gene: EHMT2 (a.k.a. BAT8, C6orf30, G9A, GAT8, KMT1C, NG36)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 2O8J http://www.thesgc.org/structures/2O8J/