AD-003
(Plasmid #25502)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC (Addgene plasmid # 26094)
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: AD-003
• Alt name: PDB IDs: 2EX4, 5E1B, 5E1D, 5E1M, 5E1O, 5E2A, 5E2B
• Species: H. sapiens (human)
• Insert Size (bp): 666
• Entrez Gene: NTMT1 (a.k.a. AD-003, C9orf32, HOMT1A, METTL11A, NRMT, NTM1A)
• Promoter: T7
• Tags / Fusion Proteins:
  • 6x His (N terminal on backbone)
  • TEV cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

For more information, please see:
http://www.thesgc.org/structures/2EX4/
http://www.thesgc.org/structures/5E1B
http://www.thesgc.org/structures/5E1D
http://www.thesgc.org/structures/5E1M
http://www.thesgc.org/structures/5E1O
http://www.thesgc.org/structures/5E2A
http://www.thesgc.org/structures/5E2B