SIRT5
(Plasmid #25487)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SIRT5
• Alt name: PDB ID: 2B4Y, 2NYR (supersedes 2FZQ)
• Alt name: Sirtuin 5 isoform 1
• Alt name: SIR2L5
• Species: H. sapiens (human)
• Insert Size (bp): 807
• Entrez Gene: SIRT5 (a.k.a. SIR2L5)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB ID: 2B4Y, 2NYR (supersedes 2FZQ) https://www.rcsb.org/structure/2NYR
SGC links:
https://www.thesgc.org/structures/2B4Y/
https://www.thesgc.org/structures/2NYR/