SHMT2
(Plasmid #25479)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: Structural Genomics Consortium Clones (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Backbone size w/o insert (bp): 7328
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SHMT2
• Alt name: PDB ID: 6DK3 (supersedes 3OU5 and 2A7V)
• Alt name: Mitochondrial Serine Hydroxymethyltransferase
• Species: H. sapiens (human)
• Insert Size (bp): 1464
• Mutation: A269T
• Entrez Gene: SHMT2 (a.k.a. GLYA, HEL-S-51e, NEDCASB, SHMT, mSHMT)
• Tag / Fusion Protein:
  • His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 6DK3 (supersedes 3OU5 and 2A7V) https://www.rcsb.org/structure/6DK3
SGC: http://www.thesgc.org/structures/2A7V/