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Addgene

Actin-53b-ires-EGFP
(Plasmid #25459)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25459 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Beta-actin/CMV-IRES-EGFP
  • Backbone manufacturer
    home made
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BAF53b
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1485
  • Entrez Gene
    Actl6b (a.k.a. Actl6, ArpNa, Baf53b)
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer gttattgtgctgtctcatc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Actin-53b-ires-EGFP was a gift from Jerry Crabtree (Addgene plasmid # 25459 ; http://n2t.net/addgene:25459 ; RRID:Addgene_25459)

  • For your References section:

    Regulation of dendritic development by neuron-specific chromatin remodeling complexes. Wu JI, Lessard J, Olave IA, Qiu Z, Ghosh A, Graef IA, Crabtree GR. Neuron. 2007 Oct 4. 56(1):94-108. 10.1016/j.neuron.2007.08.021 PubMed 17920018

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