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Addgene

pCIS-krPAM1
(Plasmid #25456)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25456 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PAM1
  • Alt name
    Peptidylglycine alpha-amidating monooxygenase
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2400
  • Entrez Gene
    Pam (a.k.a. PHM)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIS-krPAM1 was a gift from Richard Mains (Addgene plasmid # 25456 ; http://n2t.net/addgene:25456 ; RRID:Addgene_25456)

  • For your References section:

    Expression of individual forms of peptidylglycine alpha-amidating monooxygenase in AtT-20 cells: endoproteolytic processing and routing to secretory granules. Milgram SL, Johnson RC, Mains RE. J Cell Biol. 1992 May . 117(4):717-28. 10.1083/jcb.117.4.717 PubMed 1577852
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