PHMcc(delta16 PHM 356s)
(Plasmid
#25455)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 25455 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCIS
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePHM
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Alt namePeptidylglycine alpha-hydroxylating monooxygenase
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Alt namePAM
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1000
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MutationContains the catalytic core of PHM, including aa 42-356. aa 26- 41 are deleted
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Entrez GenePam (a.k.a. PHM)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bsp106 (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PHMcc(delta16 PHM 356s) was a gift from Betty Eipper (Addgene plasmid # 25455 ; http://n2t.net/addgene:25455 ; RRID:Addgene_25455) -
For your References section:
Peptidylglycine alpha-hydroxylating monooxygenase: active site residues, disulfide linkages, and a two-domain model of the catalytic core. Kolhekar AS, Keutmann HT, Mains RE, Quon AS, Eipper BA. Biochemistry. 1997 Sep 9. 36(36):10901-9. 10.1021/bi9708747 PubMed 9283080
Map uploaded by the depositor.
