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Addgene

pIB2
(Plasmid #25451)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 25451 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2500
  • Vector type
    Pichia pastoris expression
  • Selectable markers
    HIS4

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pichia pastoris HIS4
  • Species
    Pichia pastoria
  • Insert Size (bp)
    2532
  • Entrez Gene
    PAS_chr1-4_0160

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site BglII (destroyed during cloning)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIB2 was a gift from Benjamin Glick (Addgene plasmid # 25451 ; http://n2t.net/addgene:25451 ; RRID:Addgene_25451)

  • For your References section:

    A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris. Sears IB, O'Connor J, Rossanese OW, Glick BS. Yeast. 1998 Jun 15;14(8):783-90. 10.1002/(SICI)1097-0061(19980615)14:8<783::AID-YEA272>3.0.CO;2-Y PubMed 9675822
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