pSSec7-mGFPx3
(Plasmid #25449)

• Depositing Lab: Benjamin Glick
• Publication: Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 7700
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Sec7
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 6000
• Mutation: GFP contains A206K for monomerization.
• Entrez Gene: SEC7 (a.k.a. YDR170C)
• Tag / Fusion Protein:
  • mGFPx3 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry