YIPlac204-TC-Sec7-6xDsRed-M1
(Plasmid #25448)

• Depositing Lab: Benjamin Glick
• Publication: Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

Backbone

• Vector backbone: YIPlac204
• Backbone size w/o insert (bp): 8300
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Sec7
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 6000
• Entrez Gene: SEC7 (a.k.a. YDR170C)
• Tag / Fusion Protein:
  • 6xDsRed.M1 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry